Study on Positive Control for GM Papaya (55-1) Detection Method by GUS (.BETA.-Glucuronidase) Assay
نویسندگان
چکیده
منابع مشابه
A β-glucuronidase (GUS) based cell death assay.
We have developed a novel transient plant expression system that simultaneously expresses the reporter gene, β-glucuronidase (GUS), with putative positive or negative regulators of cell death. In this system, N. benthamiana leaves are co-infiltrated with a 35S driven expression cassette containing the gene to be analyzed, and the GUS vector pCAMBIA 2301 using Agrobacterium strain LBA4404 as a v...
متن کاملApplication of an improved glucuronidase assay method to the study of human blood beta-glucuronidase.
Previous conditions in which phenolphthalein glucuronide was employed as substrate (1) have proved unsatisfactory for the determination of glucuronidase in plasma, serum, and laked blood cells. Thus, low glucuronidase activities such as frequently occur in plasma could not be accurately measured or even approximated, owing to a combination of circumstances; i.e., too short an incubation period ...
متن کاملbeta-Glucuronidase (GUS) transposons for ecological and genetic studies of rhizobia and other gram-negative bacteria.
A series of transposons are described which contain the gusA gene, encoding beta-glucuronidase (GUS), expressed from a variety of promoters, both regulated and constitutive. The regulated promoters include the tac promoter which can be induced by IPTG, and nifH promoters which are symbiotically activated in legume nodules. One transposon contains gusA with a strong Shine-Dalgarno translation in...
متن کاملA functional screen identifies lateral transfer of beta-glucuronidase (gus) from bacteria to fungi.
Lateral gene transfer (LGT) from prokaryotes to microbial eukaryotes is usually detected by chance through genome-sequencing projects. Here, we explore a different, hypothesis-driven approach. We show that the fitness advantage associated with the transferred gene, typically invoked only in retrospect, can be used to design a functional screen capable of identifying postulated LGT cases. We hyp...
متن کامل[Comparison of carotenoid components between GM and non-GM papaya].
We compared the carotenoid profile in GM papaya (Sunup) line to that of a non-GM one (Sunset). First, to identify major carotenoids in papaya, large-scale extraction was carried out with methanol. HPLC analyses of the methanol extracts revealed that both papayas mainly contained 5 pigments and no apparent difference was observed in the HPLC profiles. On the basis of LC/MS data and photodiode-ar...
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ژورنال
عنوان ژورنال: Journal of the Food Hygienic Society of Japan (Shokuhin Eiseigaku Zasshi)
سال: 2005
ISSN: 0015-6426,1882-1006
DOI: 10.3358/shokueishi.46.55